Organoid Kit
Human Gastric Organoid Construction Kit
SKU: GBOK026
Product Specifications
| SKU | GBOK026 |
|---|---|
| Product Name | Human Gastric Organoid Construction Kit |
| Category | Organoid Kit |
| Size / Format | Kit contains: Tissue Storage Solution (25mL), Tissue Rinsing Solution (250mL), Tissue Fixation Solution (5mL), Digestion Solution I (30mL), Organoid Washing Solution (250mL), Red Blood Cell Lysis Solution (5mL), Rinsing Solution (5mL), Trypan Blue (100μL), Matrigel (1mL), Organoid Culture Medium (60mL), Supplement I (500μL, 100x), Organoid Recovery Solution (25mL), Digestion Solution II (10mL), Digestion Solution III (10mL), Organoid Cryopreservation Solution (25mL). Consumables: Sterile Ophthalmic Scissors, Sterile Ophthalmic Forceps, Sterile Centrifuge Tube (1.5mL x10), Sterile Pasteur Pipette (3mL x15), Sterile Cell Strainer (100μm x2), Suspension Cell Culture Plate (24 holes x2), Sterile Cryovial (2mL x10) |
| Storage Condition | The Organoid Culture Medium should be stored below -20°C and has a shelf life of 6 months. It can be stored at 2-8°C for two weeks. Matrigel: -20°C, avoid repeated freeze-thaw, aliquot as needed upon first use. Supplement I: -20°C, avoid repeated freeze-thaw. Most other reagents: 2-8°C. |
| Shelf Life | See components |
| Application | Organoid construction, tissue processing, drug screening, disease modeling, biobanking |
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Product Description
The 'One-stop' Organoid Construction Kit developed is an all-in-one solution for organoid construction, integrating tissue sampling, organoid culturing, subculturing, cryopreservation, and resuscitation. It is characterized by its user-friendly nature and ready-to-use design, achieving a remarkably high success rate of over 90% in organoid construction. This product equips customers with all the necessary consumables and reagents throughout the entire experimental process, minimizing the time consumption on non-experimental procedures.
Applications
Human Gastric Organoid Construction Kit enables multiple applications in biomedical research and drug development:
1. **Disease Modeling**: Establish patient-derived organoid models that faithfully recapitulate tumor heterogeneity, histopathological features, and genomic profiles of original tissues for studying cancer biology, progression mechanisms, and therapeutic resistance.
2. **Drug Screening & Development**: High-throughput evaluation of drug efficacy, toxicity, and combination strategies using physiologically relevant 3D models. Supports testing of chemotherapeutics, targeted therapies, immunotherapies, and novel drug candidates.
3. **Precision Medicine**: Generate patient-specific organoid models for predicting individual treatment responses, enabling personalized therapeutic strategy selection and companion diagnostic development.
4. **Basic Research**: Study tissue development, stem cell biology, cell-cell interactions, organogenesis mechanisms, and disease pathogenesis in controlled 3D environments.
5. **Biobanking**: Establish and maintain organoid lines for long-term storage, distribution, and collaborative research through standardized cryopreservation protocols.
6. **Regenerative Medicine Research**: Explore potential applications of organoid-derived cells in tissue repair and transplantation approaches.
7. **Multi-omics Studies**: Compatible with downstream genomic, transcriptomic, proteomic, and metabolomic analyses for comprehensive molecular characterization.
1. **Disease Modeling**: Establish patient-derived organoid models that faithfully recapitulate tumor heterogeneity, histopathological features, and genomic profiles of original tissues for studying cancer biology, progression mechanisms, and therapeutic resistance.
2. **Drug Screening & Development**: High-throughput evaluation of drug efficacy, toxicity, and combination strategies using physiologically relevant 3D models. Supports testing of chemotherapeutics, targeted therapies, immunotherapies, and novel drug candidates.
3. **Precision Medicine**: Generate patient-specific organoid models for predicting individual treatment responses, enabling personalized therapeutic strategy selection and companion diagnostic development.
4. **Basic Research**: Study tissue development, stem cell biology, cell-cell interactions, organogenesis mechanisms, and disease pathogenesis in controlled 3D environments.
5. **Biobanking**: Establish and maintain organoid lines for long-term storage, distribution, and collaborative research through standardized cryopreservation protocols.
6. **Regenerative Medicine Research**: Explore potential applications of organoid-derived cells in tissue repair and transplantation approaches.
7. **Multi-omics Studies**: Compatible with downstream genomic, transcriptomic, proteomic, and metabolomic analyses for comprehensive molecular characterization.
Instructions for Use
**Complete Workflow Protocol**:
**Phase 1: Tissue Collection & Transport**
- Collect tissue samples using provided instruments
- Place immediately in Tissue Transport Solution
- Transport at 4°C within 24-48 hours
**Phase 2: Tissue Processing**
- Rinse tissue with Tissue Rinsing Solution
- Mince into small fragments using sterile scissors
- Digest with Digestion Solution I for 30-60 minutes at 37°C
- Filter through cell strainer to obtain single cells or small clusters
**Phase 3: Organoid Establishment**
- Count viable cells using Trypan Blue staining
- Mix cells with pre-chilled Matrigel (on ice)
- Plate as domes in pre-warmed 24-well plate
- Incubate at 37°C for 10-15 minutes to solidify
- Overlay with complete Organoid Culture Medium
**Phase 4: Culture & Expansion**
- Change medium every 2-3 days
- Monitor organoid formation under microscope
- Passage when organoids reach optimal density (typically 7-14 days)
- Use Digestion Solution II for mechanical/enzymatic dissociation
**Phase 5: Cryopreservation**
- Harvest organoids at desired passage
- Suspend in Organoid Cryopreservation Solution
- Freeze gradually at -80°C, then transfer to liquid nitrogen
**Key Notes**: All procedures require aseptic technique. Maintain Matrigel on ice at all times.
**Phase 1: Tissue Collection & Transport**
- Collect tissue samples using provided instruments
- Place immediately in Tissue Transport Solution
- Transport at 4°C within 24-48 hours
**Phase 2: Tissue Processing**
- Rinse tissue with Tissue Rinsing Solution
- Mince into small fragments using sterile scissors
- Digest with Digestion Solution I for 30-60 minutes at 37°C
- Filter through cell strainer to obtain single cells or small clusters
**Phase 3: Organoid Establishment**
- Count viable cells using Trypan Blue staining
- Mix cells with pre-chilled Matrigel (on ice)
- Plate as domes in pre-warmed 24-well plate
- Incubate at 37°C for 10-15 minutes to solidify
- Overlay with complete Organoid Culture Medium
**Phase 4: Culture & Expansion**
- Change medium every 2-3 days
- Monitor organoid formation under microscope
- Passage when organoids reach optimal density (typically 7-14 days)
- Use Digestion Solution II for mechanical/enzymatic dissociation
**Phase 5: Cryopreservation**
- Harvest organoids at desired passage
- Suspend in Organoid Cryopreservation Solution
- Freeze gradually at -80°C, then transfer to liquid nitrogen
**Key Notes**: All procedures require aseptic technique. Maintain Matrigel on ice at all times.
Notes
- For research use only. Not for diagnostic or therapeutic procedures.
- All products undergo rigorous quality testing including sterility and performance validation.
- Contact our support team for protocol optimization and troubleshooting assistance.
Related Keywords
Human Gastric Organoid Construction Kitorganoidgastrictissue
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