Mouse Organoid Differentiation Medium
Mouse Intestinal Organoid Differentiation Medium
Catalog Number: GBCM0067
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Product Specifications
| Product Name | Mouse Intestinal Organoid Differentiation Medium |
|---|---|
| Size | 100mL |
| Quality Control Standards | pH: 7.2~7.4Endotoxin content: <10 EU/mLBiosafety: Negative for bacteria, fungi, and mycoplasma detectionPerformance test: Passed organoid culture test |
| Storage Condition | Store at -20℃,valid for 6 months.Store at 2-8℃valid for 2 weeks.Once thawed, use immediately. Do not refreeze. Can be aliquoted and stored according to your needs. |
| Precautions | Upon receipt, store immediately in a -20°C freezer, or aliquot and store in a 2-8°C refrigerator.When unpacking, check for any leakage or damage.Use aseptic techniques during product handling to avoid contamination. |
Product Description
Cell Source and Tissue Characteristics:
Mouse intestinal organoid differentiation medium is used to differentiate intestinal stem cell organoids into mature intestinal epithelial cell types.The mouse small intestine/colon organoids are derived from Lgr5+ intestinal stem cells, and the stem cell state is maintained under amplification conditions.After differentiation, four main intestinal epithelial cell types are produced: (1) enterocytes-expressing sucrose isomaltase (SI), alkaline phosphatase (ALPI), and Villin; (2) goblet cells-expressing Muc2 and TFF3; (3) enteroendocrine cells-expressing chromophilic granuloprotein A (ChgA/Chromogranin A); (4) Paneth cells (unique to the small intestine)-expressing chromophilic granuloprotein A (ChgA/Chromogranin A); (4) Paneth cells (unique to the small intestine)-expressing chromophilic granuloprotein A (ChgA/Chromogranin A). Lysozyme (Lyz1), Defensins (Defensins).The Wnt signal withdraw is the core driving force for initiating differentiation.The differentiation system is suitable for nutrient absorption research, drug transport, intestinal infection and inflammatory bowel disease modeling.
Mouse intestinal organoid differentiation medium is used to differentiate intestinal stem cell organoids into mature intestinal epithelial cell types.The mouse small intestine/colon organoids are derived from Lgr5+ intestinal stem cells, and the stem cell state is maintained under amplification conditions.After differentiation, four main intestinal epithelial cell types are produced: (1) enterocytes-expressing sucrose isomaltase (SI), alkaline phosphatase (ALPI), and Villin; (2) goblet cells-expressing Muc2 and TFF3; (3) enteroendocrine cells-expressing chromophilic granuloprotein A (ChgA/Chromogranin A); (4) Paneth cells (unique to the small intestine)-expressing chromophilic granuloprotein A (ChgA/Chromogranin A); (4) Paneth cells (unique to the small intestine)-expressing chromophilic granuloprotein A (ChgA/Chromogranin A). Lysozyme (Lyz1), Defensins (Defensins).The Wnt signal withdraw is the core driving force for initiating differentiation.The differentiation system is suitable for nutrient absorption research, drug transport, intestinal infection and inflammatory bowel disease modeling.
Mouse Intestinal Organoid Differentiation Medium is a high-performance complete medium specifically designed for Mouse Intestinal Organoid culture. This advanced formulation employs optimized nutrient composition and growth factor cocktails to precisely mimic the in vivo tissue microenvironment, supporting long-term establishment, expansion, and maintenance of organoids from primary tissue samples.
Mouse Intestinal Organoid Differentiation Medium is suitable for multiple applications including precision medicine research, high-throughput drug screening, toxicity testing, developmental biology studies, and organoid biobanking. The product undergoes rigorous quality control including sterility testing, endotoxin detection, functional validation using established organoid lines, and batch-to-batch consistency checks.
Mouse Intestinal Organoid Differentiation Medium is suitable for multiple applications including precision medicine research, high-throughput drug screening, toxicity testing, developmental biology studies, and organoid biobanking. The product undergoes rigorous quality control including sterility testing, endotoxin detection, functional validation using established organoid lines, and batch-to-batch consistency checks.
Notes
- For research use only. Not for diagnostic or therapeutic procedures.
- All products undergo rigorous quality testing including sterility and performance validation.
- Contact our support team for protocol optimization and troubleshooting assistance.
- More validation data are prepared for uploading.