Human Organoid Complete Medium
Human Cholangiocarcinoma Organoid Complete Medium
Catalog Number: GBCM0006
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Product Specifications
| Product Name | Human Cholangiocarcinoma Organoid Complete Medium |
|---|---|
| Size | 100mL |
| Quality Control Standards | pH: 7.2~7.4Endotoxin content: <10 EU/mLBiosafety: Negative for bacteria, fungi, and mycoplasma detectionPerformance test: Passed organoid culture test |
| Storage Condition | Store at -20℃,valid for 6 months.Store at 2-8℃valid for 2 weeks.Once thawed, use immediately. Do not refreeze. Can be aliquoted and stored according to your needs. |
| Precautions | Upon receipt, store immediately in a -20°C freezer, or aliquot and store in a 2-8°C refrigerator.When unpacking, check for any leakage or damage.Use aseptic techniques during product handling to avoid contamination. |
Product Description
Cell Source and Tissue Characteristics:
Cholangiocarcinoma (CCA) is a highly malignant tumor that originated from the epithelial cells of the bile duct and is divided into intrahepatic bile duct cancer (iCCA) and extrahepatic bile duct cancer (eCCA).iCCA originated in the intrahepatic bile duct system, while eCCA includes hepatic portal bile duct cancer and remote bile duct cancer.The organoid of bile duct cancer mainly comes from the tumor tissue obtained by the patient's surgical resection or biopsy.This type of organoid retains the histological characteristics of the primary tumor (such as positive expression of CK7, CK19, and KRT19), gene expression profile, and genomic landscape, including common driver mutations such as KRAS, TP53, SMAD4, IDH1/2, and FGFR2 fusion.Compared with hepatocellular carcinoma organoid, bile duct carcinoma organoid is more dependent on R-spondin 1, but usually does not require Wnt3a and Noggin to maintain growth.Some iCCA organoids can be self-sustained without exogenous Wnt signals, reflecting the autocrine activation of the Wnt pathway in tumors.
Cholangiocarcinoma (CCA) is a highly malignant tumor that originated from the epithelial cells of the bile duct and is divided into intrahepatic bile duct cancer (iCCA) and extrahepatic bile duct cancer (eCCA).iCCA originated in the intrahepatic bile duct system, while eCCA includes hepatic portal bile duct cancer and remote bile duct cancer.The organoid of bile duct cancer mainly comes from the tumor tissue obtained by the patient's surgical resection or biopsy.This type of organoid retains the histological characteristics of the primary tumor (such as positive expression of CK7, CK19, and KRT19), gene expression profile, and genomic landscape, including common driver mutations such as KRAS, TP53, SMAD4, IDH1/2, and FGFR2 fusion.Compared with hepatocellular carcinoma organoid, bile duct carcinoma organoid is more dependent on R-spondin 1, but usually does not require Wnt3a and Noggin to maintain growth.Some iCCA organoids can be self-sustained without exogenous Wnt signals, reflecting the autocrine activation of the Wnt pathway in tumors.
Human Cholangiocarcinoma Organoid Complete Medium is a high-performance complete medium specifically designed for Human Cholangiocarcinoma Organoid culture. This advanced formulation employs optimized nutrient composition and growth factor cocktails to precisely mimic the in vivo tissue microenvironment, supporting long-term establishment, expansion, and maintenance of organoids from primary tissue samples.
Human Cholangiocarcinoma Organoid Complete Medium is suitable for multiple applications including precision medicine research, high-throughput drug screening, toxicity testing, developmental biology studies, and organoid biobanking. The product undergoes rigorous quality control including sterility testing, endotoxin detection, functional validation using established organoid lines, and batch-to-batch consistency checks.
Human Cholangiocarcinoma Organoid Complete Medium is suitable for multiple applications including precision medicine research, high-throughput drug screening, toxicity testing, developmental biology studies, and organoid biobanking. The product undergoes rigorous quality control including sterility testing, endotoxin detection, functional validation using established organoid lines, and batch-to-batch consistency checks.
Notes
- For research use only. Not for diagnostic or therapeutic procedures.
- All products undergo rigorous quality testing including sterility and performance validation.
- Contact our support team for protocol optimization and troubleshooting assistance.
- More validation data are prepared for uploading.